<p>It is tough to detect 5F-MDMB-PICA in biological matrices due to its fast metabolic fee in vivo. In this work, 5F-MDMB-PICA metabolic profiles were investigated by liquid chromatography--quadrupole exactive excessive area orbitrap mass spectrometer (LC-Q Exactive HF MS), with correct mass measurements in human urine, serum and pubic hair. To get hold of intact metabolites, solid-phase