Four-aco-met
Approximately 200 mg of sodium sulfate was added to every tube to suppress emulsions and the tubes were centrifuged for one more 5 min at 2,400 g. About 1.zero N hydrochloric acid (three.5 mL) was added and the tubes were blended for 30 min. The tubes have been then centrifuged for 5 min at 2,400 g before the highest natural layer was aspirated to waste. The remaining aqueous portio